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Considering the T m values and binding affinities of all PV2 and PV3 mutants, PV2-S26pAcF, PV2-S71PAcF, PV3-S85pAcF and PV3-N77pAcF which showed significantly improved stability and binding affinity, were selected for 2 times 2 combination, resulting in four pairs of BsNbs

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An increasing number of disorders are associated with reduced NO synthesis or elevated oxidative removal of NO into the arterial wall, frequently involving those linked with risk factors for atherosclerosis such as high blood pressure, high cholesterol, heart failure, smoking, and diabetes [24]

In recent years, there has been an increasing interest in exploring this promising immunotherapy approach [369]