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Transfection of the oncogene c-RAF in rat parotid epithelial cells results in loss of TJ integrity, downregulation of occludin and claudin-1, and altered ZO-1 and E-cadherin localization

A real time-PCR analysis was conducted using SYBR Premix-Ex TagTM (Takara, Dalian, China) on a Roche LightCycler 480 System (Roche Applied Science, Penzberg, Germany)

2019 Mar 18;20(6):1351

Sample collection After eight weeks, the rats in the control and experimental groups were fasted overnight for 1618 h with free access to water to reduce the post-prandial variability of liver enzyme activities