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10.1210/jcem.79.5.7962341 4 AshpoleN

Spleen tyrosine kinase Syk is necessary for E-selectin-induced L2 integrin-mediated rolling on intercellular adhesion molecule-1

Immunoblotting Cells were washed with PBS at the end of treatments if applicable, and then lysed with an appropriate volume of 2X Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) with 5% -mercaptoethanol (Bio-Rad Laboratories)