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Drug Metab Dispos (2001) 29(11):14928

Materials and Methods Bacterial strains, growth media, and culture conditions Bacterial strains, plasmids, and primers used in this study are shown in Tables S1S4

Crystal structure and characterization of pyrroloquinoline quinone disodium trihydrate

Table 1 PCR reaction system Components Volume(L Final concentration 2 High-Fidelity PCR Master Mix (With Dye)* 25 1 Template*** x - Forward Primer10 mol/L** 2 0.4 mol/L Reverse Primer10 mol/L** 2 0.4 mol/L ddH2O Up to 50 - Note: 1) *The 1 final concentration of the mix contains 1 U/50 L of polymerase, 2 mmol/L Mg2+, and 200 mol/L dNTPs