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Figure 3 By influencing metabolic pathways, KD can also indirectly modulate NAD + /NADH levels, which play an important role in maintaining redox homeostasis (127)

doi: 10.1016/j.celrep.2015.10.021 233 SammsRJSmithDPChengCCAntonellisPPPerfieldJWKharitonenkovAet al

A selenophosphate synthetase assay using selenide-bound PRDX6 as a selenium source was performed by incubating 200 g of PRDX6 WT or C47S with 2 mM sodium selenite and 20 mM DTT in degassed 20 mM Tris-HCl (pH 7.5) buffer at room temperature for 30 min

Orchidectomy worsened the effect of hepatic I/R while testosterone treatment improved the hepatic I/R-induced functional and morphological disruption in both intact and testosterone-depleted rats, owing to its antioxidant, anti-inflammatory, and anti-apoptotic properties, which were exerted partly via its direct action, and via being converted to estradiol