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( b, e, f, i, j ) The intensity of HyPerRed fluorescence of synchronized AHR or NRF2 knockout MCF-7 and U2OS cells ( b ), synchronized U2OS cells treated with Linrodostat (20 M) or Epacadostat (20 M) ( e ), synchronized IDO1 knockout or IDO1 overexpression-U2OS cells ( f ), and synchronized MCF-7 and U2OS cells transfected with si-IDO2 or si-TDO2 ( i, j ) were detected by flow cytometry for 48 h

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e ) Alternative codons AGAN, AUAN, CCCN, CGAN, and AGUN were tested through transplantation into the Spe qtRNA Pyl UAGA MG02 scaffold in a tRNA-mRNA selection using 4 g/ mL chloramphenicol plates