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Specifically, gut microbiota dysbiosis, such as increased abundance of Proteobacteria and Actinobacteria and decreased numbers of Bacteroidetes , Prevotella and Firmicutes phyla , reduces the expression of tight junction protein genes, directly leading to the impairment of intestinal barrier function, making harmful microbiota and microorganisms pass through the intestinal barrier, stimulating the immune system and inducing immune cell inflammation, and ultimately accelerating NAFLD and liver fibrosis ( Klebsiella pneumoniae and Escherichia coli under anaerobic conditions can increase the activity of the cytochrome P450 2E1 enzyme, resulting in an increase in reactive oxygen species and free radicals, thus leading to oxidative damage and necrosis of liver cells ( According to recent reports, numerous TCMs, including Si Miao Formula [composed of Phellodendron chinense Schneid, Atractylodes lancea (Thunb.), Coix lacryma-jobi L