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Additional added variable plots depicting pS6 induction in children or adults of low or high severity grade are shown in Supplemental Figure 4E

Selenium Why It Works: Selenium is a trace mineral essential for the formation of selenoproteins, which protect developing sperm from oxidative damage

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After the treatment period, the culture medium of each well was replaced by 0.2 ml of calcein AM solution (Sigma-Aldrich) at 2 M in phosphate buffer [PBS 1X] and the culture plates were put into the incubator for 30 minutes before fluorescence reading using a Synergy H1 microplate reader (Biotek, Winooski, VT, USA