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TM3 cells were harvested after treatment, stained with Annexin V-FITC and PI reagent, and incubated in the dark for 15 minutes

Ongoing signaling stops once you stop applying, which is why most users treat GHK-Cu as long-term maintenance

To measure the reverse reaction rates, we mixed a pre-equilibrated mixture of RT (20 M) and GSH (10 or 5 mM) with an equal volume of PBS and followed the disappearance of RT-GSH fluorescence (Fig

g,h,i , Dehydratase activity towards ultrafiltered flavan-3,3,4-triol ( 4 ) and GSH-transferase activity towards CDNB ( 5 ) of variants of Pt GSTF8 ( g ), Ph AN9 ( h ), Vv GST4 ( i ) measured in colorimetric 96-well-plate assays