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In vivo protein degradation For time-course of RGL2 degradation after GA application, 5-day-old seedlings of 35S:MYC-RGL2 , 35S:MYC-RGL2 dwa1-1 , 35S:MYC-RGL2 35S:Flag-DWA1 , 35S:MYC-RGL2 35S:PYL4-GFP , 35S:MYC-RGL2 35S:Flag-PYL11 , and 35S:MYC-RGL2 pyr1 pyl1 pyl2 pyl4 seedlings were treated with 10 M PAC for 60 min followed by 10 M GA 3 for 30 or 60 min