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This includes confirmed low serum vitamin B12 levels, pernicious anaemia, malabsorption states such as coeliac disease, inflammatory bowel disease, or post-gastric or bariatric surgery, unexplained anaemia, neuropathy or neurological symptoms consistent with vitamin B12 deficiency, a long-term vegan diet with symptoms or low levels, or failure of oral vitamin B12 supplementation

So that's going to bind to the calcium and not count that

Calculate backward from your protocol endpoint: how many total administrations, at what dose, over how many days

Oncoimmunology 7:e1391972 Ellerby HM, Arap W, Ellerby LM, Kain R, Andrusiak R, Rio GD, Krajewski S, Lombardo CR, Rao R et al (1999) Anti-cancer activity of targeted pro-apoptotic peptides