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TMT ratio check The combined and dried ratio check sample was re-dissolved in Buffer A (5% CH 3 CN, 95% water, 0.1% formic acid, 20 L) and desalted using C18 stage tips prepared in-house using 3 C18 discs (3 M Empore) stacked in 200 L pipette tips

They were then permeabilized in 0.2% Triton X-100 for 10 min and blocked with 8% goat serum for 1 h at room temperature

[DOI: 10.1056/NEJMra051884] [DOI] [PubMed] [Google Scholar] 45.Boyle AJ, Sweeney RM, McAuley DF

The available evidence suggests that such biotechnological methods not only increased the quantity and availability of PPs for industrial, food and medicinal purposes (360, 381, 382), but also expedited the number of scientific investigations conducted on PPs to find the most potent metabolites for large-scale applications (361)