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After adding EnVision Flex 3,3-diaminobenzidine tetrahydrochloride (DAB, K346811-2, Agilent, Santa Clara, CA, USA) for 5 min at room temperature to visualize GPx3, the slides were washed with tap water, counterstained with hematoxylin and mounted in Pertex

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Peptide therapy involves the use of specific peptidesshort chains of amino acids that act as building blocks for proteinsto target various physiological functions within the body

Hence, OoCs are increasingly used to study disease mechanisms, discover biomarkers, and test therapeutic candidates with improved accuracy compared to traditional cell cultures or animal models ( Figure 1 )