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To generate cell lysate overexpressing the appropriate AGO variant, 200 ml of Expi293F cells at about 2 million per ml cell density were transfected with 190 g of the AGO expression plasmid, 10 g of pMaxGFP (Lonza) and 600 g of polyethylenimine (Polysciences, 23966) incubated in 10 ml of Opti-MEM at room temperature for 20 min