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therefore, the use of population controls might have been more appropriate

10.1111/j.1744-7909.2007.00583.x 82 LundeC.BaumannU.ShirleyN

The column temperature was consistently maintained at 70 C, with a sample injection volume of 1 l

Then, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.02% Triton-X-100/1 phosphate-buffered saline (PBS) and blocked in a blocking fluid (1 PBS + 10% fetal bovine serum (FBS) and 1% bovine serum albumin (BSA)) (Thermo Fisher Scientific, Waltham, USA)