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The glycine cleavage system is essentially reversible but catalyzes glycine synthesis significantly only under anaerobic conditions, such as in anaerobic bacteria or anaerobic systems in vitro supplemented with NADH+H + [32]

Tetrahydrobiopterin (BH 4 ) plays a vital role in this reaction by stabilizing the eNOS dimer and preventing the formation of superoxide, a potentially harmful byproduct, ensuring NO synthesis proceeds efficiently (Xia et al., 1998a

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In this case, the R 2 value is calculated as 0.9828, indicating that the model accounts for approximately 98.28% of the variation observed in the data