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1 10 4 cells were collected and analyzed by a BD FACS Celesta flow cytometer (BD Biosciences, San Jose, USA)

For instance, in AD mice, mitochondrial damage increases mtDNA leakage into the cytosol, where it engages cGAS and activates STING, driving microglial secretion of type I interferons and pro-inflammatory cytokines that contribute to neuronal dysfunction [93]

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That preservative is the entire reason BAC water exists it inhibits the growth of bacteria, which means a single vial can be punctured multiple times over weeks without becoming a contamination risk