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TMPK and CMPK activity assay by NADH-coupled enzymatic assay Activity assay was measured using the spectrophotometric method by coupling ADP formation to NADH oxidation catalyzed by pyruvate kinase and lactate dehydrogenase as described previously 20

In general, payload release occurs in the TME or after cellular internalization within endosomal/lysosomal compartments, and in some systems may further proceed following endosomal escape, thereby determining the cytosolic availability of the active degrader [184,185,186]