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Lpez-Daz D, Delgado-Notario JA, Cleric V, Diez E, Merchn MD, Velzquez MM

Sleep improvements typically appear within 2-4 weeks, while body composition and performance changes develop over 2-3 months, with continued optimization through 6 months

To examine whether the administration of the Si-based agent alleviates the visceral pain and discomfort associated with UC, we investigated the neuronal activities in the nuclei of the dorsal medulla oblongata [the nuclei of the solitary tract (NST) and the dorsal vagal nuclei (DVN)], and the central amygdala (CeA) using immunostaining for c-Fos, a neuronal active marker

Real time Quantitative PCR Method RNA was extracted using the RNeasy Mini kit (QIAGEN, 74106)