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X100-500ML, Sigma-Aldrich), 10% Horse Serum (26050-070, Gibco) in phosphate buffer) with the primary antibodies (Iba1 (AB48004, Abcam), GFAP (Z0334, Dako), rhodopsin (MAB5316, Merck Millipore), cone arrestin (AB15282, Merck Millipore) for three days at room temperature

In North Carolina, a BAC of 0.15% or more is considered gross impairment and can result in harsher penalties, including the requirement of an ignition interlock system on your car

ChIPseq data processing Reads were aligned to the mm10 reference genome with bowtie 100 version 0.12.9 with parameters --n 2 and --m 1 to keep reads with multiple alignments in one position

If using strong exfoliants or retinoids, alternate days at first or apply vitamin C in the morning and those actives at night