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Furthermore, when cells were pre-cultured in in DMEM supplemented with FBS that had been depleted of biotin using streptavidin, FLAG-tagged RELA became detectable in the absence of added biotin but was markedly diminished upon biotin supplementation (Supplementary Figure S4A)

517 Energy metabolism in T2DM patients In T2DM, energy metabolism is disrupted, which is characterized by chronic hyperglycemia and impaired carbohydrate, fat, and protein metabolism

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Dynamic alterations in monocyte numbers, subset frequencies and activation markers in acute and convalescent COVID-19 individuals