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2014 Dec;102(6):1518-27 (View) Mora-Esteves C, Shin D

After ultrasonic disruption, cells were centrifuged at 15,000 rpm for 20 min at 4 C

Estimation of glutathione Plant extracts (TCA extract, 0.5 mL) were mixed with H 2 O (500 L), 0.2 M phosphate buffer (pH 7.0, 500 L) and 50 mM 5, 5-dithiobis (2-nitrobenzoic acid) (DTNB 100 L) and incubated for 30 min