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Additionally, it bolsters the immune system, reduces inflammation, and may contribute to improved skin health

Oxidising agents used were H 2 O 2 (03 mM), menadione (060 M) and diamide (01.75 mM)

Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)