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For instance, the efficiency of NC-AA is low due to the non-compatibility of structurally diverse NC-AA with ribosomes and elongation factors ( in vitro including ATP synthesis (Kurihara et al., 2011), PURE protein component self-regeneration (Lavickova, Laohakunakorn, and Maerkl, 2020), and ribosomal components (Jewett et al., 2013)