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Description

Purification of GST-tagged proteins For protein expression, 400 mL bacterial cultures (DH5 or BL21 Codon Plus RIPL) were transformed with the corresponding expression plasmids, grown in LB medium supplemented with appropriate antibiotics and induced with 0.1 mM IPTG at OD 600 = 0.81.0

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TB-500 is the synthetic version used therapeutically

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coli displays resistance to sublethal pressure treatments (Malone et al., 2006)

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(19) Cystic fibrosis leads to suppressed glutathione levels and the impaired ability to produce more at a normal rate

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