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These data suggest that the purified antibodies from the lupus patients are polyclonal and that the B-CLL and normal donor antibodies were monoclonal, thus accounting for the different banding patterns

For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

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Unextracted plasma was directly subjected to EIA, demonstrating displacement curves parallel to the standards