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The target sequence GTGTCAGGAGGTGTTTGGAAAG (SEQ ID NO: 2) was inserted into pSUPER vector (Oligoengine, Seattle WA) which drives endogenous production of shRNA under the HI promoter

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This triple blend addresses tissue regeneration from multiple angles: BPC-157 optimizes vascularization and reduces inflammation, TB-500 facilitates cellular migration and tissue organization, and GHK-Cu promotes extracellular matrix synthesis and activates genes associated with tissue remodeling and repair

The culture media was substituted by those including DMSO or ZIL and the cells were cultured until the control sample (which does not contain the solvents) became 80% confluent