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Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)

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These effects may potentially provide a new therapeutic solution for stroke with specific beneficial effects on the CNS (i.e., reperfusion, amelioration of neuronal damage leading to recovery in the absence of memory, motor, and coordination deficits, and expression of specific genes in the hippocampus)