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Ferritin-1 ameliorated the decreased cell viability and elevated LDH caused by GPx-4 gene knockout

M.MoinA.AnwarS.Osman ElkhalifaA

Different amounts of caspase proteins (100 g for caspase 3 and 200 g for caspase 9) were diluted in 250 l reporter lysis buffer and incubated with equal volumes of 2x caspase reaction buffer containing 100 M fluorogenic substrates for caspase 3 or 9 (Calbiochem) at 37 C for 3 hours