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The enzymatic components of the anti-oxidative defense system consist of a number of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX) and the enzymes of ascorbate-glutathione (AsA-GSH) cycle namely, ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) (Mittler et al., 2004

A total volume of 1 ml was adjusted with sample buffer (10 mM potassium phosphate buffer pH 7.4, 3 mM KCl, 140 mM NaCl, and 6 mM D-glucose)

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