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The relatively simple measurement, inexpensive instrumentation, and robustness and potential for high-throughput assay make AuNP-based colorimetric assays excellent candidates for in vitro measurements of enzyme activity

Borgna-Pignatti C, Vergine G, Lombardo T, Cappellini MD, Cianciulli P, Maggio A, et al

The ANLS switches on glutamate released by neuronal terminals and then taken up by astrocytes via the excitatory amino acid transporters 1 and 2 (EAAT1 and 2) to convert into glutamine or glutathione, which is activated by a gradient