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RNA isolation and real-time quantitative reverse-transcriptase PCR (qRT-PCR) analysis For RNA extraction, the peqGold RNA isolation kit (Peqlab, Erlangen, Germany) was used for phLF and the RNeasy Mini Plus Kit (Quiagen

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In recent years, the standard temperature for performing xenograft assays in the literature has been increased to 34C at the expense of shortening the incubation time to 3 to 6 days post-injection (dpi) (108) systematically evaluated morphological abnormalities and developmental effects in injected and control embryos at multiple time points

However, it is possible this may have occurred since this group was eating less at week 8 than at baseline regarding kcal/kg and protein/kg