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Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1

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Using a rat model of DMBA-induced mammary tumors, it is shown that the use of garlic powder successfully inhibited the growth of tumors and validated the fact that DADS has been more active than AMS and DAS

RNA was reverse transcribed in a final volume of 20 l containing 1 RT buffer (500 mM each dNTP, 3 mM MgCl2, 75 mM KCl, 50 mM Tris-HCl pH 8.3), 10 units of RNasinTM ribonuclease inhibitor (Promega, Madison, #N2511, WI, USA), 10 mM dithiothreitol, 50 units of Superscript II RNase H- reverse transcriptase (Gibco-BRL, #18064014, Gaithersburg, MD, USA), 1.5 mM random hexamers (Pharmacia, Uppsala, Sweden) and 1 g of total RNA