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Materials and methods Experimental animals The animal experiments were performed on the basis of ethical regulations for animal testing, and the animal protocol employed in this study was approved by the Animal Experimentation Committee of Jinan University (IACUC-20191212-02)

Pathogenesis Arises from fat-storing perisinusoidal cells, so-called Ito cells

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Overproduction of reactive oxygen species (ROS) in the nucleus pulposus cells (NPCs) is a critical contributor to the progression of IDD through increasing oxidative stress in the microenvironment of the extracellular matrix (ECM) 5 , leading to ferroptosis and long-term persistent inflammation 6,7 , which would cause the loss of structure and function in the intervertebral disc (IVD) ultimately