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The fluorescence intensity ratios (405 nm/488 nm) obtained under these conditions were used to calculate the degree of oxidation of roGFP2 (OxD roGFP2 ) and subsequently convert it to E GSH using the Nernst equation (Eq

And higher doses have also occasionally been linked to eye issues that do not occur at lower levels

10.3389/fpubh.2023.1206283 85 RoughanW

Subsequently, H 2 O 2 was added at the required concentration and the fluorescence response was monitored using a BMG Labtech CLARIOstar fluorescence plate-reader