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MG reacts with arginine, lysine, and cysteine residues in proteins, resulting in the formation of advanced glycation end products (AGEs), which often leads to protein inactivation [7,9]

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After washing with demineralized water, the sections underwent an antigen retrieval step in microwave-warmed (300 W) 0.1 M Tris/hydrochloride (HCl) buffer (pH 9.0), for 15 min, followed by a cooling-down period at room temperature (RT)