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The PCR condition was as follows: initial denaturation at 94 C for 2 min, followed by 30 cycles of denaturation at 98 C for 10 s, annealing at 55 C for 30 s, and extension at 68 C for 40 s

In vitro ATP binding assays were performed with recombinant GST-PDK1 WT and K163R proteins purified from E

Martinez-Valbuena et al., 2019), and decreased insulin signaling in the brain can inhibit phosphatidylinositol 3-kinase/Akt and activate GSK-3 (Ma et al., 2015)