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RNA extraction and RT-qPCR RNA extraction from the hippocampus and neurosphere was performed using TRIzol RNA isolation reagents (15596026, Thermo Fisher Scientific) and either the RNeasy Mini or Micro kit (74104 and 74004, Thermo Fisher Scientific)

PLoS ONE 6:e27746 Miniarikova J, Evers MM, Konstantinova P (2018) Translation of MicroRNA-based Huntingtin-lowering therapies from preclinical studies to the clinic

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