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Advise patients and caregivers: how to treat with the overdose reversal agent in the event of an opioid overdose to tell family and friends about the opioid overdose reversal agent, and to keep it in a place where family and friends can access it in an emergency to read the Patient Information (or other educational material) that will come with their opioid overdose reversal agent

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The cells were then rinsed three times with PBS solution, incubated with cell lysis buffer for 30 min, scraped off, and centrifuged at 12,000 rpm for 15 min at 4 C