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Mass spectrometry analysis was performed on a Q Exactive hybrid quadrupole Orbitrap tandem mass spectrometer (ThermoFisher Scientific) in positive ion mode by direct infusion of total extracts

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Leupeptin that inhibits proteases within lysosomes and autophagosomes 20 was applied with glutamate and NH 4 Cl, but the expression of GS was still reduced (Supplementary Fig