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Composed of three amino acidsglutamate, cysteine, and glycineit is the most abundant non-protein thiol in mammalian cells, where it exists in two primary states: the reduced form (GSH) and the oxidized form, glutathione disulfide (GSSG)

Leucine: This essential amino acid is involved in protein synthesis, wound healing, blood sugar control and metabolism

As an electrophilic chemical, ITCs might react covalently with the thiol groups of (proteins and) glutathione in plants, similar to what has been demonstrated in different in vitro systems 38,77,78 as well as in insects feeding on GSL or ITC diets 79,80

In some classes this residue is a tyrosine while in some other is a serine or a cysteine