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These units run a multidisciplinary clinic for children with DRE which means failure of two or more well selected and proper dosed AED to achieve sustained seizure freedom 2

Substrate uptake studies Uptake of glucose and palmitate were assessed with fluorescent analogues 2-( N -(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, Waltham, MA, USA, N13195), Bodipy FL C12 (D3822) and Bodipy FL C16 (D3821) (Thermo Fisher Scientific, Waltham, MA, USA) using flow cytometry (MACS Quant, Miltenyi Biotec, Bergisch Gladbach, Germany)

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