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Using saccharin as a prototype sweetener at a dose corresponding to the ADI for humans (5 mg/kg per day), the authors further characterised the faecal microbiota composition of saccharin-consuming mice and demonstrated that saccharin induced strong dysbiosis, with an increase in the number of bacteria from the Bacteroides genus and the Clostridiales order

Although inflammation of the terminal ileum is common in Crohn disease and ulcerative colitis, some studies report that vitamin B12 deficiency is uncommon in these populations (30), whereas others report rates of vitamin B12 deficiency as high as 14% among patients with Crohn disease (30

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The pharmacokinetic rationale is questionable given the 5-day half-life, but some users report better side effect tolerance with split dosing