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7 Chang, C

E) Representative images depicting EdU cell-cycle analysis after 3 h (A549) and 6 h (H358) when cells attached between experimental groups

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Then the membrane was blocked with 5% BSA for 1 h at room temperature, followed by incubating with primary antibodies and IRDye800 flurophore-conjugated antibodies (LI-COR Biosciences, Lincoln, NE, USA)