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Patients had a median age of 31.5 years (2077 years) and were primarily identified as black (90%), male (70%), and had an Eastern Cooperative Oncology Group (ECOG) performance status of 1 (60%)

To screen the mutants for LCA-induced biofilm formation, individual colonies were picked using sterile wooden round toothpicks and inoculated into 2 mL YCFA in deep-well 96-well plates (Corning, catalog no