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Eight week old female and male mice, after anaesthetization with intraperitoneal injection of ketamine hydrochloride (100 mg/kg body weight, Thea), xylazine (10 mg/kg body weight, VMD), and the local anesthetic (Unicane 0.4%

10.3390/pharmaceutics16040491 50 DerakhshanZ.FerranteM.TadiM.AnsariF.HeydariA.HosseiniM

Histopathological examination and lesion scoring Following necropsy, brain tissue specimens (cerebrum and cerebellum) were obtained from different rat groups ( n = 8 per group), rinsed in physiological saline solution (NaCl 0.9%), and then placed at least for 24 h in 10% neutral buffered formalin (pH 7.4)