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Amplification conditions were optimized for each primer pair, and melt-curve analysis was performed at the end of the reactions to confirm amplification specificity

Its solid character is stable, its aqueous solution is easy oxidized in the air for oxidized glutathione, widely exists in baker's yeast, wheat germ, animal liver, chicken blood, pig blood, tomato, pineapple, cucumber, of which is highest in the wheat germ and liver, content as high as 100~1000 mg/100 g

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Table 3 summarises the known overexpressed CYPs and their associated insecticide resistance in Aedes mosquitoes