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Our findings further support the induction of ferroptosis through the detection of proteins xCT, GPX4, and SREBP1

Most of our patients benefit from combining two or three of these mechanisms in a structured plan rather than chasing one modality alone

Escherichia coli strains NEB 5-alpha, BL21(DE3), BL21 Star (DE3) (New England Biolabs, Frankfurt, Germany) or M15 (Qiagen, Hilden, Germany) were used as hosts for propagation of respective vectors and for production of recombinant His-tagged proteins were grown at 37 C in yeast tryptone (YT) broth containing 50 g/ml ampicillin (Carl Roth GmbH, Karlsruhe, Germany) or 100 g/ml streptomycin (Merck)

FAD absorption spectroscopy To assess the presence of protein-bound FAD, 100 g of recombinant WT or D285N mutant FSP1 protein or 10 g of free FAD control was resuspended in a total volume of 200 l in 50 mM sodium phosphate pH 7.4