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A broader analysis revealed over 2,700 regions with reduced methylation in cancer and over 1,500 regions that curcumin remethylated, highlighting its ability to restore a more normal epigenetic state ( Figure 1 To assess the impact of curcumin on DNA methylation, another study used three CRC cell linesa harbor cancer therapy group cell line 116 (HCT116), a human tumor cell line 29 (HT29), and RKOwhich were exposed to curcumin treatment ( Epithelialmesenchymal transition (EMT) is a key process in CRC progression and metastasis, characterized by the loss of epithelial markers (e.g., E-cadherin) and the gain of mesenchymal markers (e.g., vimentin and ZEB1), enhancing cancer cell migration and invasion ( 3.1.3 Histone modification effects Recent research also shows that histone modifications can be regulated by CUR (Figure 1)

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