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10.1161/CIRCRESAHA.119.316453 444 TangY.LuoH.XiaoQ.LiL.ZhongX.ZhangJ.et al (2021b)

Histopathological evaluation using H&E staining (Figure 11A) revealed that neurons in the Ischemia-Reperfusion group were disorganized, exhibited contracted and irregular cell bodies, and displayed pyknotic nuclei with indistinct structural features

In various embodiments, the extraneous thiol is present in the reaction in a stoichiometric ratio relative to the amino acid comprising conjugation partner of from about 200:1 to about 0.05:1, 100:1 to 0.05:1, 80:1 to 0.05:1, 60:1 to 0.05:1, 40:1 to 0.05:1, 20:1 to about 0.05:1, 10:1 to about 0.5:1, 5:1 to about 1:1, or 3:1 to about 1:1

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